This clinical trial focuses on studying allergies related to Blomia tropicalis, which is a type of house dust mite that commonly causes allergic reactions in certain geographic regions. The study aims to determine the standardized strength of an allergic extract used for diagnosis of this specific allergy. The treatment being tested is a solution for skin-prick test containing different concentrations of Blomia tropicalis allergen extract.

The study involves using various concentrations of the allergen extract through intraepidermal application, which means the solution is applied just under the surface of the skin. During the testing, histamine dihydrochloride will be used as a positive control substance, and sodium chloride (salt water) as a negative control to ensure accurate results. The skin’s reaction to these substances will be measured and compared.

The purpose of this research is to establish the correct concentration of the allergen extract that produces a skin reaction comparable to a standard allergic response. This will help determine the proper strength of the extract for future use in allergy testing. The study will also monitor any unwanted reactions that might occur during the testing process to ensure the safety of the procedure.